This interactive group session will give an introduction to flow cytometric data analysis and will provide an introductory guide to the use of the analytical software Kaluza. Attendees should ideally have performed some flow cytometry already or be familiar with the use of flow cytometry for example through attendance of the ‘Introduction to Flow Cytometry Course’. The session requires access to a working copy of the analytical software Kaluza –  install and access details can be found here: Kaluza Data Analysis Software. During the session attendees will work through and analyse example data, which will be provided, and students own data can be discussed.  There will also be a discussion of the appropriate statistical tools and considerations for presenting flow cytometry data. 

Learning Outcomes. 

• Ability to open Flow Cytometry Standard (FCS) files in Kaluza and produce appropriate data plots including dot plots, histograms and overlays. 
• Understanding of the 3 principle ways Kaluza can be used to analyse and present data; protocol duplication mode, composite mode and data batches.  
• Appreciation of fundamental principles of data analysis required for peer review publications including, consistency of approach, data quality and ability to describe objective and subjective outputs. 
• Understanding of key statistics that can be extracted and how they may be used to represent data for publication. 

Booking Conditions 

Privacy Statement 

The Researcher Academy is committed to protecting the privacy of researchers who register for our training courses and events. Your completion of the booking process shall constitute your consent to the collection, use and disclosure of your personal data to University staff and external partners responsible for the delivery of the course or event for which you have registered.  Personal information shared includes; name, role/position, mode of study, School/Department, dietary and accessibility requirements. 

Latecomer policy 

Researchers should plan to arrive prior to the advertised course start time. Except for exceptional reasons, there will be no admittance to a researcher academy or Faculty Training Programme (FTP) course 15 minutes after the advertised course start time. 

Importance of booking commitment 

When booking on to a Researcher Academy short course you are entering into a commitment to attend. If you find that you are no longer available to attend you MUST cancel your place (on the system if more than three days before the course) or if at short notice by emailing ra-training@nottingham.ac.uk. This will ensure that your place can be offered to another researcher on the waiting list. Failure to cancel a place results in other researchers missing out on places through the waiting list process. 

It is unacceptable for researchers to just not attend when booked onto a course. The Researcher Academy maintains records of those who repeatedly do not attend courses they have booked. This may affect future eligibility to book onto further Researcher Academy courses and will affect considerations for Researcher Academy funded opportunities. 

Booking Instructions/ Information 

This online course/Webinar is delivered by the Researcher Academy 

Our webinars/ online courses are very popular, especially in the current period and we are experiencing large volumes of people on our waiting lists.  

We appreciate that personal circumstances are changing daily during the University’s lock-down period but please do let us know if you would like to cancel your place on this course so we can offer your place to another delegate. 

Please note, attendances for webinars/ online courses will still be recorded and corresponding training points, when shown as available, will be awarded.   

Accessing the course 

To access the course please follow the link below and log into Moodle using your normal username and password. You must use the link below when accessing this course for the first time as it allows you to self-enrol onto the course. Make sure that you log in to the course within two days of the course start date, after this date enrolment is disabled. 

Assessment and Training Points 

For specific information on how to submit the course assessment in order to receive training points/attendance badge please read the ‘assessment' section on the course Moodle page. If you have any problems or queries, please don't hesitate contact:pg-training@nottingham.ac.uk 

To access all the course materials and the courses live streaming link visit the course Moodle page.

Pre-task:

You will need to spend approx 30 minutes before the start of the webinar (10am prompt) downloading the Kaluza software onto your Windows laptop/PC (please note the software does not run on MacOS). Depending on your internet speed/PC/laptop this could take longer, so please plan accordingly. If you encounter problems you can join the webinar to seek advice from the tutor, but this task must at least be attempted before the webinar.

Please visit course's Moodle page in advance of the session to familiarise yourself with the content: The moodle page is accessible below.