This interactive group session will give an introduction to flow cytometric data analysis and will provide an introductory guide to the use of the analytical software Kaluza. Attendees should ideally have performed some flow cytometry already or be familiar with the use of flow cytometry for example through attendance of the ‘Introduction to Flow Cytometry Course’.

The session requires access to a working copy of the analytical software Kaluza –  install and access details can be found here: Kaluza Data Analysis Software. During the session attendees will work through and analyse example data, which will be provided, and students own data can be discussed.  There will also be a discussion of the appropriate statistical tools and considerations for presenting flow cytometry data. 

Learning Outcomes. 

• Ability to open Flow Cytometry Standard (FCS) files in Kaluza and produce appropriate data plots including dot plots, histograms and overlays. 
• Understanding of the 3 principle ways Kaluza can be used to analyse and present data; protocol duplication mode, composite mode and data batches.  
• Appreciation of fundamental principles of data analysis required for peer review publications including, consistency of approach, data quality and ability to describe objective and subjective outputs. 
• Understanding of key statistics that can be extracted and how they may be used to represent data for publication. 

Course Accessibility

The following table shows a summary of what is needed to participate in the course. 

If you feel you will experience any difficulties participating, please let us know via the ‘special requirements’ tab, providing as much information as possible. The special requirements tab can be completed when you book your place. Alternatively, you can contact us directly at ra-training@nottingham.ac.uk.

Access Moodle
Access MS Teams
Access and/or complete self-study/ pre-course tasks prior to main session(s)
Attend the live session at the specified date and time
Use MS Teams chat box function during the live session
Engage with online materials
Watch and listen to the course tutor(s) and/or other attendees during the live session
Follow presentation slides during the course during the live session
Take part in group activities/ discussions

Booking Conditions 

Our webinars/ online courses are very popular, especially in the current period and we are experiencing large volumes of people on our waiting lists.  

Please do let us know if you would like to cancel your place on this course so we can offer your place to another delegate. 

Please note, attendances for webinars/ online courses will be recorded. There are no training points associated with courses run by the Researcher Academy

Pre-requisites:

Introduction to Flow Cytometry short course or prior hands-on experience using a Flow Cytometry experimental set up.  

IMPORTANT INFORMATION! The Kaluza software used during this online course needs a Windows operating system and cannot be downloaded to MacOS .  In order to participate in the course you must have access to this, and complete the pre-session tasks, below: 

Pre-task:

You will need to spend approximately 30 minutes before the start of the webinar (10am prompt) downloading the Kaluza software onto your Windows laptop/PC (please note the software does not run on MacOS). Depending on your internet speed/PC/laptop this could take longer, so please plan accordingly. If you encounter problems you can join the webinar to seek advice from the tutor, but this task must at least be attempted before the webinar.

Please visit course's Moodle page in advance of the session to familiarise yourself with the content: The moodle page is accessible below.